Development of bacterial artificial chromosome library resources for parasitoid Hymenoptera (Nasonia vitripennis and Nasonia giraulti: Pteromalidae)

TitleDevelopment of bacterial artificial chromosome library resources for parasitoid Hymenoptera (Nasonia vitripennis and Nasonia giraulti: Pteromalidae)
Publication TypeJournal Article
Year of Publication2010
AuthorsMunoz-Torres, M. C., C. A. Saski, B. Blackmon, J. Romero-Severson, and J. H. Werren
JournalInsect Mol Biol
Volume19 Suppl 1
Pagination181-7
Date PublishedFeb
ISBN Number1365-2583 (Electronic)09
Accession Number20167027
KeywordsAnimals Chromosomes, Artificial, Bacterial/*genetics DNA Fingerprinting DNA Primers/genetics *Gene Library Genome, Insect/*genetics Genomics Species Specificity Wasps/*genetics
Abstract

The species of the genus Nasonia possess qualities that make them excellent candidates for genetic and genomic studies. To increase the wealth of genomic resources for the genus we constructed publicly available bacterial artificial chromosome (BAC) libraries for Nasonia vitripennis and Nasonia giraulti. Libraries have 36 864 clones each, empty-vector contents of approximately 2% and average insert sizes of 113.1 and 97.7 Kb, respectively, representing 12 and 11 genome equivalents. The N. vitripennis library was used for genome sequence assembly and in efforts at positional cloning of a developmental gene. The genome assembly of N. vitripennis is currently composed on 6181 un-joined scaffolds. These BAC libraries can be used to identify and close regions between scaffolds of the genome assemblies of both species.

URLhttp://www.ncbi.nlm.nih.gov/pubmed/20167027