Genomic Serivces

454 / Illumina Sequencing

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CUGI works with several external partners to deliver 454 and Illumina sequencing.

Capillary Fragment Sizing

CUGI offers capillary fragment sizing of fluorescent labeled fragments on our ABI 3130xl or ABI3730xl DNA Analyzers. Please contact CUGI for an exact quote for available spectrals and custom projects.

DNA Hybridization

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CUGI employs DNA hybridization using radioisotopes and BAC macroarrays to identify corresponding genomic regions in BAC libraries. We offer single probes and custom pools up to 125 markers at a time. This is an invaluable tool to identify genes or regions of interest and integrate genetic information into a physical context. Please contact CUGI for an exact quote for a project.

High Information Content Fingerprinting

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CUGI is one of a few labs that specializes in physical mapping by High-Information Content Fingerprinting (HICF). The technique uses 5 restriction enzymes, fluorescent labeling of fragments and fragment sizing via capillary electrophoresis. We also specialize in contig assembly/analysis, and employ a unique marker pooling strategy to anchor genetic information to a physical framework. Collectively, an integrated physical/genetic framework provides a superhighway for structural, functional, and evolutionary genomics research.

Sanger Sequencing

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CUGI offers high quality Sanger-style sequencing with fast turnaround times. Our services range from DNA isolation from host cells to sequencing of purified plasmids and PCR products. We operate on both high and low throughput platforms and can accommodate single samples to large scale sequencing projects. Our ABI sequencers offer superior quality and long reads. We also offer a suite of sequence assembly and analysis options, please see our options in our Bioinformatics section for a list of services. Sequencing orders may be placed through our online ordering system.