Cell Sorter

Previously offered through CU-CAL (Clemson University Cell Analysis Laboratory), CUGI now offers high-speed cellular analysis and sorting services using BD FACScan and Cytopeia Influx instruments.

Characterisitcs of the Cytopeia Influx

• 50,000 events/second
• Up to 32 parameters with 4-decade dynamic range
• Rare event detection
• Sterile workplace
• Adaptable for immunology, oceanography and cell cycle analysis
• Small particle detector for bacterial studies
• Flo Jo Software for analysis

Helpful Hints

• The following controls are needed for all experiments.
• Unstained cells to measure autofluorescence
• Isotype controls for all antibodies
• Single-color tubes for all fluorochromes used in multicolor combinations
• Other controls as specified by core staff
• Cells should be analyzed the same day as they are stained unless they are fixed (e.g. in 1% formaldehyde). Cells should be in medium (unfixed) for viable sorting
• Stained cell should be kept on ice until analyzed or sorted

Please contact CUGI for an exact quote for a project.