Sample Prep for DNA Sequencing

Please use the following guide when preparing samples for sequencing at CUGI

• High Copy Plasmids Bacterial Culture: Please grow an overnight culture in 96-well format in LB+15% glycerol. Seal plate with a clear plate sealer (not foil type), freeze to -80C and ship on dry ice
• Low Copy Plasmids Bacterial Culture: Please grow an overnight culture in 96-well format in LB+15% glycerol. Seal plate with a clear plate sealer (not foil type), freeze to -80C and ship on dry ice
• Purified High Copy Plasmids: Please send purified plasmid DNA at a concentration of 30-50ng either dried down or resuspended within 3µl
• Purified Low Copy Plasmids: Please send purified plasmid DNA at a concentration of 150-200ng either dried down or resuspended within 5µl
• Purified PCR Products: Samples must be purified of unincorporated nucleotides and residual primers. RULE OF THUMB: For each kb of fragment size send approximately 10ng either dried down or resuspended within 3µl of ddH20.
• Ready to Load Samples: Send samples dried down in 96-well format. CUGI will resuspend in HiDi, denature, and load on ABI sequencer

Sample Delivery
On-Campus Customers
Please drop off during the following times on the 3rd floor across from spiral staircase

Monday-Friday 9:00-9:30am
Monday-Thursday 2:30-3:00pm

Off-Campus Customers
please ship to:

Clemson University Genomics Institute
CUGI Sequencing Center BRC 310
105 Collings Street
Clemson, SC 29634

Additional Information
Other questions and answers related to DNA sequencing at CUGI can be found on our FAQ.