Protocols

Hybridization Protocol for BAC Colony Filters

0.5 M Sodium Phosphate, pH7.2
134 g Na2HPO4.7H2O in 850 ml H2O
pH to 7.2 with 4 ml 85% H3PO4
adjust to 1 L with H2O

Macroarray Filter Illustration

Standard CUGI Macroarray Filter Layout

Click the image to see the macroarray filter row column address patterns.

Sample Prep for DNA Sequencing

sequencer_3730xl.jpg
Please use the following guide when preparing samples for sequencing at CUGI
  • High Copy Plasmids Bacterial Culture: Please grow an overnight culture in 96-well format in LB+15% glycerol. Seal plate with a clear plate sealer (not foil type), freeze to -80C and ship on dry ice (For on-campus customers: plate out on a petri dish so that I can pick single colonies)
  • Low Copy Plasmids Bacterial Culture: Please grow an overnight culture in 96-well format in LB+15% glycerol.