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High information content fingerprinting HICF) technique, based on capillary electrophoresis and the SNaPshot labeling kit (Luo et al., 2003. Genomics, 82(3): 378-389), is used to construct physical maps for both M. guttatus and M. lewisii. Both BAC libraries of M. guttatus, each containing 36,864 BAC clones, have been fingerprinted (96.2% and 95.2% success rate, respectively). The M. lewisii library with 36,864 clones has also been fingerprinted (98% success rate). To integrate the genetic and physical maps, gene-based markers are being anchored to all three Mimulus BAC libraries using an overgo pooling strategy. These two physical maps are also available at http://www.mimulusevolution.org/.
Clones: 56,624, Contigs: 2,535, Markers: 582, Singletons: 18,287
Physical Map Release Date: September 2, 2008 (eighth release)
Due to tremendous ecological and phenotypic diversity, the genus Mimulus (Scrophulariaceae, order Lamiales) has been widely used as a superb model flowering plant system for ecological and evolutionary genetics. All species are hermaphroditic and self-fertile, facilitating the construction of permanent populations such as inbred lines and recombinant inbred lines (RIL). M. guttatus species complex (2n = 28) and M. lewisii (2n = 16) are two established study systems. Both display tremendous variation in traits directly related to reproductive isolation in the wild, including habitat speciation, floral divergence, and the fertility of hybrids. They generate the shared material and infrastructure necessary for community-wide comparative studies within Mimulus and among flowering plants.