What is High Copy and Low Copy Sequencing

CUGI uses copy number information to determine the quantity of sequencing reagents and template concentration needed for a sequencing reaction.

High copy refers to a high copy number of plasmids per cell (>300) and PCR amplicons. Typically, high copy plasmids contain inserts 10kb in size or less. Examples of high copy plasmids are pGEM, TOPO, pBlueskript, etc. Low copy refers to a low copy number of plasmids per cell (1-20). Typically, these are BACs, fosmids, and some expression vectors.