Physical Mapping
The CUGI Physical Mapping group constructs physical maps of both entire
genomes or small regions of interest. DNA fingerprinting is performed using either
the High Information Content Fingerprinting (HICF) method utilizing capillary electrophoresis
or traditional agarose gel electrophoresis. After electrophoresis, fragment analysis is
performed using either GeneMapper(ABI), GenoProfiler, FP Pipeliner and FP Miner (Bioinforsoft)
or Image programs. Fragment data is then analyzed in FPC (Soderlund) to construct the physical
map. Marker hybridization and sequence data can be added to the FPC project to enhance the
quality of the physical map and help integrate genetic and physical maps. Services include
HICF, agarose fingerprinting and hybridization. Please contact us for more information and pricing.
Services
Please click on each service for further details.
The physical mapping group offers High Information Content Fingerprinting (HICF) in a
96-well format. The technique uses 5 restriction enzymes, fluorescent labeling of
fragments and fragment sizing done on an Applied Biosystems 3730 DNA analyzer.
Software utilized to analyze the data include the most recent versions of GenoProfiler,
FP Miner, FP Pipeliner, GeneMapper and FPC.
Agarose Fingerprinting in a 96 well or single sample format using a single
restriction enzyme, fragment sizing is done an a agarose gel. Software used to
analyze the data includes Image and FPC.
Marker hybridization using macroarrays on nylon filters is done either a single
marker at a time or by pooling multiple markers. The marker data can be added to
the physical map to help integrate the genetic and physical maps.
Physical Mapping data analysis is done utilizing control clones to insure as
accurate results as possible. Agarose fingerprinting uses Image and FPC, while
the HICF uses GeneMapper, GenoProfiler, FP Miner, FP Pipeliner and FPC.
