A plant (D/R1/4/1/7) showing the bi-pistil trait was first observed during 2004 amongst M. truncatula plants (cv. Jemalong, 2HA), transformed (Agrobacterium mediated) with Alfalfa mosaic virus (AMV) coat protein gene in negative orientation (CP-), which had been generated by Jayasena et al (2001). Self-fertilised seed of this plant was collected. The progeny of this plant was grown and all showed the bi- pistil trait. As all the progeny plants of the bi-pistil plant identified exhibited the trait, it was confirmed that the trait was heritable and the plant could be classified as a mutant. We propose the name bi- pistil mutant, bip for the mutation. In comparison to the wild type plants, the bip plant showed normal phenotypes for all other traits studied. Of the two pistils, one assumed normal development and developed into a normal shaped pod, while the second pistil showed much reduced size and rate of growth.
Jayasena, KW, Hajimorad, MR, Law, EG, Rehman, A-U, Nolan, KE, Zanker, T, Rose, RJ, Randles, JW. 2001. Resistance to Alfalfa mosaic virus in transgenic barrel medic lines containing the virus coat protein gene. Australian Journal of Agricultural Research 52: 67-72..