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Mimulus
BAC-End Sequencing
BAC-end sequencing (BES) of M.
guttatus and M. lewisii, in both the forward and
reverse directions, is the third phase of the CUGI Mimulus project.
We have constructed
libraries of both M. guttatus (MG__Ba and MG__Bb)
and M. lewisii (ML__Ba).
In this phase we performed BAC-end sequencing of the clones comprising
the minimum tile
of the physical maps of both
species. These sequences are being used to aid in the construction of the genomic map available
at the Mimulus Resource
Center. All successful sequences have been submitted to the Genbank
dbGSS database
under the accession numbers ED798518-ED806636 for M. guttatus and
ED806637-ED814994 for M. lewisii. An overall summary of the sequencing
results and downloads of successful BESs are provided to the right.
BAC-End Sequencing Status
BAC-end sequencing of the minimum tile
is complete for physical maps of both M. guttatus and M. lewisii.
Methods & Procedures
CUGI
uses the publicly available phred and crossmatch programs
to perform base calling and to remove residual vector
from sequences. We use our in-house scripts
to filter
low quality
sequences from the final data sets. Sequences with at
least 100 base pairs with a phred value of 20 or higher
and less than 5% ambiguous bases are considered successful.
All others are removed from the dataset. The final
BESs submitted to Genbank contain only the
longest contiguous non-vector sequence of bases with
low quality bases trimmed off both the beginning and
end of the sequence.
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M.
guttatus HindIII (MG_Ba)