Mimulus Home | BAC Library | Physical Map | BAC-End Sequencing | Homology | SSRs | Publications
Mimulus Project Progress
Apr 2006, M. lewisii physical map online.
Sep 2005, M. lewisii HindIII fingerprinting finished.
Sep 2005, M. gutattus physical map online.
Jun 2005, M. lewisii HindIII library fingerprinting begins.
Jan 2005, M. lewisii HindIII BAC library finished.
May 2004, M. gutattus BstyI library fingerprinting begins.
Apr 2004, M. gutattus BstyI BAC library constructed.
Dec 2003, M. gutattus HindIII library fingerprinting begins.
Sep 2003, M. gutattus HindIII BAC library constructed.
Sep 2003, Grant awarded.
 Collaborators
  Mimulus Resource Center
  Duke University
  University of Washington
   University of Montana
   Michigan State University
   UNC Chapel Hill
 Project Objectives
Construction of a complete physical map of Mimulus, which will be anchored on the molecular genetic map. The combined genetic/physical map will be made available at the Mimulus Resource Center.
 Project Phases
  BAC Library Construction
  Physical Map Construction
  BAC-End Sequencing
  Homology Analysis
  SSR Analysis
 Mimulus Resources at CUGI
  Purchase clones or filters
  M.guttatus Physical Map
  M. lewisii Physical Map
  Download Sequences
  Mimulus Homology Results
  Mined Mimulus SSRs
  FASTA and BLAST Servers
 Funding
This project is supported by NSF-FIBR grant No. 0328636 and NSF-MRI grant No. 0320635.
CUGI Mimulus Project
Welcome to the CUGI Mimulus project home page. CUGI has partnered with other research institutions to help provide a robust set of genetic resources for the Mimulus community. CUGI's role in the collaborative effort consists of BAC library construction for both Mimulus guttatus and Mimulus lewisii, construction of the Mimulus physical maps, and BAC end-sequencing to aid in the construction of the integrated physical-genetic map.

CUGI's Online Mimulus Resources
The BAC libraries are provided in the form of high-density hybridization filters and clones on a cost recovery basis for non-profit programs.All other Information generated at CUGI is made freely available to researchers for basic and applied studies in Mimulus molecular genetics. A similarity server is also available for researchers to peform BLAST or FASTA homology searches against our Mimulus sequences.

Background
Due to tremendous ecological and phenotypic diversity, the genus Mimulus (Scrophulariaceae, order Lamiales) has been widely used as a superb model flowering plant system for ecological and evolutionary genetics. All species are hermaphroditic and self-fertile, facilitating the construction of permanent populations such as inbred lines and recombinant inbred lines (RIL). M. guttatus species complex (2n = 28) and M. lewisii (2n = 16) are two established study systems. Both display tremendous variation in traits directly related to reproductive isolation in the wild, including habitat speciation, floral divergence, and the fertility of hybrids. They generate the shared material and infrastructure necessary for community-wide comparative studies within Mimulus and among flowering plants.

The exploitation of comparative genomic tools facilitates dissection of the QTLs recently identified as the key regions in speciation in the two different sections of the genus, down to the individual genes. This work will help to identify and characterize important genes underlying adaptation and speciation in plants. A detailed understanding of the molecular genetic basis of speciation will allow the testing of many long-standing hypotheses about the development of species barriers.